BBa_K812057

BBa_K812057 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K812057

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Hafez El Sayyed

Date created: 2012-09-25 11:00:00

Date modified: 2015-05-08 01:13:27

A GoldenBricked version of sfGFP protein

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K812057_sequence (Version 1)

Description

The select golden brick assembly of SfGFP was designed as a reporter that we have used to test the Goldenbrick system. On top of this, the SfGFP belongs to the family of the famous GFP family of fluorescent reporters that fits any detection purpose.

Notes

GOlden Brick Assemply Proposed by our team

Source

This DNA sequence was acquired from Alfonso Jaramillo's Lab

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Golden Brick protein prefix	4,20	engineered_region, feature/BioBrick
EcoRI	4,9	sequence_feature, feature/misc
BsaI	10,15	sequence_feature, feature/misc
XbaI	16,20	sequence_feature, feature/misc
OH3	17,20	feature/misc, sequence_feature
sfGFP	21,734	feature/cds, CDS
stop	735,740	feature/stop, stop_codon
Golden Brick protein suffix	741,765	engineered_region, feature/BioBrick
BsaI	748,753	sequence_feature, feature/misc
BbsI	741,746	sequence_feature, feature/misc
OH4	743,746	sequence_feature, feature/misc
SpeI	754,759	sequence_feature, feature/misc
PstI	760,765	feature/misc, sequence_feature

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K812057/1

Member of these Collections

• iGEM Parts Registry