BBa_K812058

BBa_K812058 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K812058

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Hafez El Sayyed

Date created: 2012-09-25 11:00:00

Date modified: 2015-05-08 01:13:27

A GoldenBricked-medium strenght RBS J61107

Details

• Types: DnaRegion
• Roles: RBS; ribosome_entry_site
• Sequences: BBa_K812058_sequence (Version 1)

Description

RBS J61107 is a medium-strength RBS belonging to a family of similar ribosome binding sites basic parts identified from a saturation mutagenic library. This part was modified to adjust to Goldenbrick assembly prrotocol

Notes

standardized goldenbrick assembly designed by our team

Source

Parts Registry

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J61107	18,29	ribosome_entry_site, feature/rbs
Goldenbrick RBS Prefix	1,17	engineered_region, feature/BioBrick
EcoRI	1,6	feature/misc, sequence_feature
BsaI	7,12	sequence_feature, feature/misc
XbaI	11,16	sequence_feature, feature/misc
OH2	14,17	sequence_feature, feature/misc
SpeI	31,36	feature/misc, sequence_feature
BsaI	37,42	feature/misc, sequence_feature
Goldenbrick RBS suffix	30,48	feature/BioBrick, engineered_region
OH3	32,35	feature/misc, sequence_feature
PstI	43,48	feature/misc, sequence_feature
spacer	30,30	feature/misc, sequence_feature

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K812058/1

Member of these Collections

• iGEM Parts Registry