BBa_K812059

BBa_K812059 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K812059

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Hafez El Sayyed

Date created: 2012-09-25 11:00:00

Date modified: 2015-05-08 01:13:27

A GoldenBricked version of week RBS J61117

Details

• Types: DnaRegion
• Roles: ribosome_entry_site; RBS
• Sequences: BBa_K812059_sequence (Version 1)

Description

J61117 is a week-strength RBS belonging to a family of similar ribosome binding sites basic parts identified from a saturation mutagenic library. This part was modified to adjust to Goldenbrick assembly prrotocol

Notes

GoldenBrick assembly developed by our team

Source

Parts Registry

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J61117	18,29	feature/rbs, ribosome_entry_site
Goldenbrick RBS Prefix	1,17	feature/BioBrick, engineered_region
EcoRI	1,6	feature/misc, sequence_feature
BsaI	7,12	feature/misc, sequence_feature
XbaI	11,16	sequence_feature, feature/misc
OH2	14,17	sequence_feature, feature/misc
spacer	30,30	feature/misc, sequence_feature
OH3	32,35	sequence_feature, feature/misc
SpeI	31,36	sequence_feature, feature/misc
PstI	43,48	sequence_feature, feature/misc
BsaI	37,42	feature/misc, sequence_feature
Goldenbrick RBS suffix	30,48	feature/BioBrick, engineered_region

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K812059/1

Member of these Collections

• iGEM Parts Registry