BBa_K812130

BBa_K812130 Version 1

Component

This part has been discontinued.

Please consider using BBa_K812030 as a replacement.

Source: http://parts.igem.org/Part:BBa_K812130

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Anna Mlynarczyk

Date created: 2012-09-19 11:00:00

Date modified: 2015-05-08 01:13:27

Citrine reporter with a Kozak sequence for expression in Xenopus

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K812130_sequence (Version 1)

Description

Citrine gene flanked by a kozak sequence for eukaryotic expression in biobrick format. This is designed to be used in mammalian and ovarion expression systems such as PCS2+ part : K812000. Ready for immediate use in Xenopus.

Notes

Kozak is included, as it cannot be added by biobrick. This part is ready for direct injection into a fertilised Xenopus tropicalis egg.

Source

Original sequence from A. victoria mutated to emit yellow light

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Kozac	1,5	feature/misc, sequence_feature
Citrine	7,720	feature/cds, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• isReplacedBy: BBa_K812030/1
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K812130/1

Member of these Collections

• iGEM Parts Registry