BBa_K812132

BBa_K812132 Version 1

Component

This part has been discontinued.

Please consider using BBa_K812032 as a replacement.

Source:
http://parts.igem.org/Part:BBa_K812132
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Mlynarczyk
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:27

mCFP with kozak sequence for expression in Xenopus



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Reporter

    Sequences BBa_K812132_sequence (Version 1)

    Description

    mCFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used with an eukaryotic expression system such as pSC2+ (K812000) and related parts.

    Notes

    Kozak is included, as it cannot be added by biobrick. This part is ready for direct injection into a fertilised Xenopus tropicalis egg.

    Source

    Original sequence form A.victoria mutated to emit blue light

    Sequence Annotation Location Component / Role(s)
    Kozac
    mCFP
    		1,5
    6,717
    		feature/misc sequence_feature
    CDS feature/cds

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    Discontinued
    igem#status
    Deleted
     
    isReplacedBy
    BBa_K812032/1
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K812132/1
     

    Attachments

    © 2018 Newcastle University, University of Utah, and collaborators
    About SynBioHub | View Source on Github | Report an Issue
    | v1.6.1 (57f00336)