BBa_K814000

BBa_K814000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K814000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn

Date created: 2012-10-02 11:00:00

Date modified: 2015-05-08 01:13:28

dehydroquinate synthase (DHQS) generator

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K814000_sequence (Version 1)

Description

Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. DHQS catalyzes the first step in the pathway, converting sedoheptulose-7-phosphate into dehydroquinate.

This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of DHQS. This part can be used to express DHQS in E. coli.

Notes

Genomic DNA was obtained from the Brett Barney Lab (University of Minnesota) for A. variabilis. From here, PCR primer extension was used to clone the ORF into a BioBrick???-compatible vector containing lacPmod, an RBS, and a multiple cloning site flanked by the BioBrick??? sites.

Source

Anabaena variabilis ATCC 29413 genomic DNA (accession NC_007413.1)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS	128,133	feature/rbs, ribosome_entry_site
DHQS	140,1373	feature/protein, CDS
lacP'	1,127	feature/promoter, promoter

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K814000/1

Member of these Collections

• iGEM Parts Registry