BBa_K814007

BBa_K814007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K814007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn

Date created: 2012-10-02 11:00:00

Date modified: 2015-05-08 01:13:28

ScyA (acetolactate synthase) generator

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K814007_sequence (Version 1)

Description

Our research has focused onnovel biosynthetic pathways for UV-protectant compounds found in two distinct algal species. One leading to the production of the unrelated UV-screening compound scytonemin was cloned from Nostoc punctiforme. ScyA (NpR1276), an acetolactate synthetase, carries out the first step in scytonemin biosynthesis, converting L-tryptophan to alpha-keto-tryptophan.

This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of scyA. This part can be used to express ScyA protein in E. coli.

Notes

N. punctiforme genomic DNA was obtained from ATCC. From here, PCR primer extension was used to clone the gene with flanking restriction digest sites to be used for vector cloning. The gene was cloned into an individual pUCBB BioBrick??? cloning vector containing a constitutive modified lac promoter (lacP???) and an RBS.

Source

Nostoc punctiforme PCC 73102 genomic DNA, accession NC_010628.1

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
lacP'	1,127	promoter, feature/promoter
RBS	128,133	feature/rbs, ribosome_entry_site
ScyA	140,1108	feature/protein, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K814007/1

Member of these Collections

• iGEM Parts Registry