Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ZHANG Zidong
Date created: 2012-09-01 11:00:00
Date modified: 2015-05-08 01:13:29
Constitutive LuxBrick Generator
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K819005_sequence (Version 1) |
Description
a fusion protein consisting of E.coli endogenous SOS system repressor LexA and fungus N.crass photosensor protein VVD with LexA carrying mutation at position 40-42 and VVD carrying mutation N56K, C71V and I135M. This part serves as an ultra sensitive photoreceptor(which can sense light as weak as moonlight) and will induce a light-dependent repression of genes with their promoter containing mutated 408 form of SOS box.Notes
LexA carrying mutation at position 40-42 and VVD carrying mutation N56K, C71V and I135M enable the sensor recognize 408 form of SOS box and with enhanced performance.Source
Construct by Peking2012.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K819008 LuxCDABEG from K325909 stop | 1,46 53,6477 6478,6480 | promoter feature/promoter sequence_feature feature/misc feature/stop stop_codon |