BBa_K820000

BBa_K820000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K820000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Philipp N. Sander
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:29

LOV2 2012



Types
DnaRegion

Roles
Coding

CDS

Description

The LOV2 protein contains a photo-activable LOV2 domain, which changes its conformation upon exposure to blue light (420-480 nm)and thus can be used to build photoswitchable fusion proteins.

This part has been prepared from an optimized LOV2 sequence published by Strickland et al. 2009.

Standard BioBrick restriction sites have been added. An nhI site has been added to facilitate fusion with fusion-compatible (non rbs-site containing) BioBricks using XbaI, which will ligate to nhI sticky ends.

Notes

2 site-directed mutagenesis PCRs were applied to remove 2 PstI sites inside of the coding sequence.

Source

A. Sativa

igem#sampleStatus No part sequence  
igem#status Planning  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K820000/1