BBa_K823015

BBa_K823015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K823015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jara Radeck

Date created: 2012-09-09 11:00:00

Date modified: 2015-05-08 01:13:30

Pxyl, a xylose inducible promoter + its regulator XylR

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K823015_sequence (Version 1)

Description

Pxyl is a promoter which is repressed by XylR. In presence of Xylose, XylR leaves the operator sequence and the Pxyl is active. XylR is present in most B. subtilis strains, so this would be a second copy of the gene.

XylR is provided with constittive promoter, RBS and terminator.

Notes

Inducible promoter, with its regulator.

Source

PCR with pXT as a template.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-35	1433,1438	promoter, feature/promoter
-10 extended	1451,1458	feature/promoter, promoter
xylR	173,1224	feature/cds, CDS
XylR-operator	1474,1496	operator, feature/operator

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K823015/1

Member of these Collections

• iGEM 2019 Cell Pxyl, a xylose inducible promoter + its regulator XylR
• iGEM 2019 Distribution Plate 6
• iGEM 2019 Distribution Plate 6 Well 1J
• iGEM 2019 Part Library
• iGEM 2019 Plasmid pSB1C3
• iGEM Parts Registry