pSBBs1C

BBa_K823023 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K823023

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jara Radeck

Date created: 2012-08-20 11:00:00

Date modified: 2015-05-08 01:13:30

pSB_Bs1C: Empty backbone for integration into Bacillus subtilis amyE locus

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_K823023_sequence (Version 1)

Description

This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the amyE locus and can be selected with Chloramphenicol (cat gene). It has a ampicillin resistance for cloning in E.coli. It originates from the Bacillus vector pDG1662.
This BioBrick is part of the LMU 2012 Bacillus BioBrick Box project: http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks

Notes

We removed any forbidden restriction sites by site-directed mutagenesis PCR.

Source

This backbone is an igem compatible version of the Bacillus vector pDG1662

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
cat	358,1008	feature/cds, CDS
amyE	1379,2407	sequence_feature, feature/misc
bla	4361,5221	feature/cds, CDS
amyE	5546,6066	feature/misc, sequence_feature

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K823023/1

Member of these Collections

• //chassis/multihost
• //chassis/prokaryote/bsubtilis
• //chassis/prokaryote/ecoli
• //plasmid/chromosomalintegration
• //plasmidbackbone/assembly
• //plasmidbackbone/operation
• iGEM Parts Registry
• igem_plasmid_vectors