BBa_K823037

BBa_K823037 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K823037

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jara Radeck

Date created: 2012-09-07 11:00:00

Date modified: 2015-05-08 01:13:30

10x His-tag (Freiburg standard+RBS)

Details

• Types: DnaRegion
• Roles: Tag; tag
• Sequences: BBa_K823037_sequence (Version 1)

Description

10xHis-tag with RBS in [[Help:Assembly_standard_25|Freiburg standard]].



prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC

suffix:ACCGGTTAATACTAGTAGCGGCCGCTGCAGT


This is a part created by the LMU-Munich 2012 team. We added five tags to the registry, all in the Freiburg standard for N-and C-terminal fusions:

[[Part:BBa_K823034|3x Flag tag]]

[[Part:BBa_K823035|HA-tag]]

[[Part:BBa_K823036|cMyc-tag]]

[[Part:BBa_K823037|His-tag]]

[[Part:BBa_K823038|Streptavidin-tag]]



Visit our project page for more usefull parts of our BacillusBioBrickBox.

Notes

We designed the part in the Freiburg standard for N-and C-terminal fusions.

Source

Gene synthesis by GeneArt.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
His	1,3	tag, feature/tag
His	4,6	tag, feature/tag
His	7,9	tag, feature/tag
His	10,12	tag, feature/tag
His	13,15	feature/tag, tag
His	16,18	feature/tag, tag
His	19,21	tag, feature/tag
His	22,24	feature/tag, tag
His	25,27	tag, feature/tag

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K823037/1

Member of these Collections

• //proteindomain/affinity
• iGEM Parts Registry