BBa_K842003

BBa_K842003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K842003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sean P. Curran, Percy Genyk, Ellen Park, Rebecca, Gao, Stephan Genyk, Megan Bernstein, Rachel Kohan, Eric Siryj, Luke Quinto
Date created: 2012-10-01 11:00:00
Date modified: 2015-05-08 01:13:33

fliA



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K842003_sequence (Version 1)

Description

fliA is an alternate sigma factor for the class 3 flagella operons. When transcribed, it forms a component of RNA polymerase sigma 28.

Used for reconstitution of flagella apparatus in E. coli B strains.

Cloning and Uses
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used in conjunction with the entire flagella synthesis genetic pathway to reproduce a complete flagella apparatus.

Notes

none

Source

fliA:Quickview ??? EcoliWiki. (n.d.). EcoliWiki. Retrieved October 1, 2012, from http://ecoliwiki.net/colipedia/index.php/fliA

Ide, N., & Kutsukake, K. (n.d.). Identification of a novel Escherichia coli gene whose e??? [Gene. 1997] ??? PubMed ??? NCBI. National Center for Biotechnology Information. Retrieved October 1, 2012, from http://www.ncbi.nlm.nih.gov/pubmed/9358034

Claret, L., Miquel, S., Vieille, N., Ryjenkov, D., Gomelsky, M., & Darfeuille-Michaud, A. A. (2007). The Flagellar Sigma Factor FliA Regulates Adhesion and Invasion of Crohn Disease-associated Escherichia coli via a Cyclic Dimeric GMP-dependent Pathway. The Journal Of Biological Chemistry, 282(46), 33275-33283. Retrieved October 1, 2012, from http://www.jbc.org/content/282/46/33275.full.pdf

Sequence Annotation Location Component / Role(s)
FliA
1,720
feature/cds CDS
igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K842003/1