Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sean P. Curran, Percy Genyk, Ellen Park, Rebecca, Gao, Stephan Genyk, Megan Bernstein, Rachel Kohan, Eric Siryj, Luke Quinto
Date created: 2012-10-01 11:00:00
Date modified: 2015-05-08 01:13:33
fliC-GFP
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K842013_sequence (Version 1) |
Description
When transcribed, RBS-fliC-GFP generates the flagellum protein bonded with a green fluorescent protein. This structure would then be exported to the growing flagella apparatus and used in the construction of the filament. When strongly expressed, this structure would directly result in the flagella filament containing GFP, allowing for the flagella to be detectable. Unfortunately we were unable to promote the sequence enough to test whether this construct actually functions in this manner.Cloning and Uses
Both the fliC were cloned via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. Due to the fact that fliC has multiple S and P restriction enzyme sites located within its DNA sequence, it was generated with XbaI sites on the forward and reverse primer. This is so as to allow for the placement of a gene promoter in the E-X sites upstream of the construct in an iGEM biobrick vector. It is important to note though that the current structure consists of the following so as to avoid the accidental loss of a gene during the cloning procedure.
Used for reconstitution of flagella apparatus in E. coli B strains.
EcoRI-XbaI-RBS-fliC-XbaI-GFP-SpeI-PstI
Notes
Used for reconstitution of flagella apparatus in E. coli B strains.EcoRI-XbaI-RBS-fliC-XbaI-GFP-SpeI-PstI
Source
fliC:Quickview ??? EcoliWiki. (n.d.). EcoliWiki. Retrieved October 1, 2012, from http://ecoliwiki.net/colipedia/index.php/fliC| Sequence Annotation | Location | Component / Role(s) |
| BBa_K842004 | 1,1497 | engineered_region feature/BioBrick |