| Types | DnaRegion
|
| Roles | engineered_region
Composite
|
Description
We added a double terminator to the intermediate part BBa_S03816 in order to make it a transcriptional unit ready to use. This part Produce LuxI and LacI signal molecules with a mCherry reporter. LuxI is a quorum sensing protein that converts SAM into acyl-homoserine lactone (AHL) thus acting as pLuxR promoter activator when bound to LuxR protein (BBa_K329007). LacI binds to the pLac regulator (BBa_R0010) and PLac01 hybrid regulator (BBa_R0011) inhibits transcription. IPTG binds to LacI and inhibits its operation, therefore promoting transcription.
Notes
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Source
BBa_S03816
