Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Leonard Sebastian Fresenborg
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:34
3-Hydroxy-3-Methyl-Glutaryl-CoA-Reductase from Yeast
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K849000_sequence (Version 1) |
Description
This is a truncated version of the 3-Hydroxy-3-Methyl-Glutaryl-Coenzyme-A-Reductase (HMG-CoA-R) from Saccharomyces cerevisiae. It catalyses the reaction of 3-Hydroxy-3-Methyl-Glutaryle-Coenzyme-A to Mevalonate which occurs in the Mevalonate Pathway to Isopentylpyrophosphate which is the Precurser of all Isoprenoides. The enzyme is naturally a membrane bound enzyme. The membrane anchor is situated N-terminal. This region is also essential for the degradational control of the enzyme. By deleting the N-terminal part to Aminoacid (inclusive) the enzyme is expected to be water soluble and deregulated.Notes
The truncation was done according to the following publication:[Pardo, Orejas: 'Enhanced Production of a Plant Monoterpene by Overexpression of the 3-Hydroxy-3-Methylglutaryl Conenzyme A Reductase Catalytic Domain in Saccharomyces cerevisiae'; Applied and Enviromental Microbiology; Vol 76 p. 6449-6454; 2010]