PfadR

BBa_K861060 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K861060

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kuanwei Sheng

Date created: 2012-09-07 11:00:00

Date modified: 2015-05-08 01:13:35

PfadR, synthetic promoter with tandem FadR binding site

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K861060_sequence (Version 1)

Description

At least to our knowledge, there is no promoter exists in nature that can respond solely to FadR since those promoters are often regulated by glucose concentration or oxidative stress and many other factors. We decided to design an artificial promoter to fulfill this task. Specifically, a tandem FadR binding site in FadL promoter is place downstream of promoter J23110 with 3 base pair overlap.
We test its function in M9 medium with oleic acid as sole carbon source in plasmid pSB6A1.

Notes

None

Source

Escherichia coli str. K-12 substr. MG1655

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
FadR tandem binding site	33,76	non_covalent_binding_site, feature/binding

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K861060/1

Member of these Collections

• iGEM Parts Registry