Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kuanwei Sheng
Date created: 2012-09-06 11:00:00
Date modified: 2015-05-08 01:13:36
Pcar, synthetic promoter repressed by CRP
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K861171_sequence (Version 1) |
Description
This is a promoter designed for the Eschaerichia coli . It includes the CRP-binding site and the RNA polymerase-binding site which have a overlap of several base pairs.So because of the steric hindrance between CRP and RNA polymerase,gene downstream of the promoter will be repressed at high concentration of CRP.In the cells ,low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream.On the contrary,high glucose concentration will result in the expression of the promoter.Notes
NoneSource
Escherichia coli str. K-12 substr. MG1655| Sequence Annotation | Location | Component / Role(s) |
| Consensus CRP binding site | 15,36 | non_covalent_binding_site feature/binding |