Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kuanwei Sheng
Date created: 2012-08-15 11:00:00
Date modified: 2015-05-08 01:13:36
FadA protein coding sequence for fatty acid degradation
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K861400_sequence (Version 1) |
Description
For a E.coli to degrade fatty acid, at least four enzymes ??? FadA, FadB, FadD, FadE and transporter???FadL are needed. Long chain fatty acids are firstly being imported by the transmembrane protein FadL. After FAs get into cells, they will be added a CoA by inner membrane-associated FadD (acyl-CoA synthase). β-oxidation is initiated by FadE(acyl-CoA dehydrogenase), which will convert acyl-CoA into enoyl-CoA. The followed cycles of hydration, oxidation, and thiolytic cleavage are carried out by tetrameric complex consisting of two FadA and two FadB monomers.This part is FadA coding sequence, which has 3-ketoacyl-CoA thiolase activity involving in the β-oxidation of living E.coli. The protein takes a part in the planning system to improve whole efficiency of fatty acid degradation. With appropriate promoters we decided, FadA expression can be under regulation of fatty acid concentration.
Notes
Before constructing the final system and testing its function, we constructed three biobrick parts with 3-ketoacyl-CoA thiolase activity (FadA, FadI, S-FadA) as candidates.Source
Escherichia coli K.12 MG1655 genome| Sequence Annotation | Location | Component / Role(s) |
| FadA coding sequence | 1,1164 | CDS feature/cds |