CBDclos(J6

BBa_K863114 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K863114
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Moritz M??ller
Date created: 2012-09-22 11:00:00
Date modified: 2015-06-17 01:20:44

CBD of C. cellulovorans cellulose binding protein gene with const. Promoter (J23100+J61101) and GFP



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K863114_sequence (Version 1)

Description

Cellulose binding Domain of Clostridium cellulovorans cellulose binding protein gene (cbp A) with a constitutive promoter and RBS (J61101) and a fused-GFP protein with His-tag.

Notes

Was made to switch from a T7-promoter to a constitutive promoter

Source

Cellulose binding domain form a cloning-vector. J61101 and GFP from the partsregistry.

Sequence Annotation Location Component / Role(s)
BBa_J23100
BBa_J61101
BBa_K863111
ATG
NgoMIV
Cellulose Binding Protein
Cellulose binding domain (CBDclos)
Cellulose Binding Protein
GS-Linker
Freiburg-Scar
BBa_K863121
BBa_E0040
His-Tag
AgeI
TAA
1,35
44,55
62,370
62,64
65,70
71,82
83,358
359,364
365,370
371,376
377,1117
377,1090
1091,1108
1109,1114
1115,1117
feature/BioBrick engineered_region
engineered_region feature/BioBrick
engineered_region feature/BioBrick
start_codon feature/start
sequence_feature feature/misc
conserved_region feature/conserved
feature/protein CDS
feature/conserved conserved_region
feature/cds CDS
feature/misc sequence_feature
feature/BioBrick engineered_region
engineered_region feature/BioBrick
tag feature/tag
sequence_feature feature/misc
stop_codon feature/stop
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K863114/1