Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erik Lundin
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:37
Low copy BioBrick temperature sensitive standard vector
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K864017_sequence (Version 1) |
Description
pSB8C15 is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and Chloramphenicol antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.Notes
The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is.The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.
Source
Based on BBa_K864001| Sequence Annotation | Location | Component / Role(s) |
| Promoter VR reverse primer binding site pSC101-ts ori VF2 forward primer binding site cmR -35 tonB terminator rrnC Lux terminator P22 late terminator T3 terminator -10 | 3235,3461 116,135 190,2405 3322,3341 2506,3168 881,886 47,115 136,175 2454,2499 3281,3321 3342,3419 903,908 | feature/promoter promoter feature/primer_binding primer_binding_site feature/misc sequence_feature feature/primer_binding primer_binding_site CDS feature/cds sequence_feature feature/misc stop_codon feature/stop feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc |