Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erik Lundin
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:37
Low copy BioBrick temperature sensitive standard vector
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K864023_sequence (Version 1) |
Description
pSB8S15(FRT) is a BioBrick standard vector with low copy pSC101ts replication origin (~5 copies per cell) and spectinomycin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The pSC101ts is a temperature sensitive pSC101 repelication origin and propagates at 30???C but stop replicating at 42???C. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.Notes
The resistance cassette is terminated by a Lux bidirectional terminator. Adjacent to this terminator there is a designed primer binding site to where the forward primer for lambda red can be designed to bind to no matter what the insert in the cloning site is or what the resistance is.The resistance cassette is flanked by SalI and SacI restriction sites for easy switching of resistance in the backbone.
The pSC101ts origin of replication is flaked by NheI and MluI restriction sites for easy switching of origin of replication.