Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ricardo Mungu??a D??az
Date created: 2012-09-25 11:00:00
Date modified: 2015-05-08 01:13:37
Signal converter, converts a HCO3- signal in a cAMP intermediate signal
| Types | DnaRegion |
| Roles | Signalling engineered_region |
| Sequences | BBa_K872000_sequence (Version 1) |
Description
This part is composed by the mild promoter J23101 with rbs B0012 attached to protein sAC and terminator B0030. Protein sAC (for soluble Adenilate Cyclase) is a 854 bp long and its function is to generate cAMP form ATP based on HCO3- concentration. This protein is involved in pH sensing, CO2 sensing and even in spermatozoa capacitance.Notes
This part is very sensitive, low concentrations of HCO3- activetes the cAMP signalitation.Source
Promoter J23101 secuence can be found in http://partsregistry.org/Part:BBa_J23101:DesignRbs B0012 secuence can be found in http://partsregistry.org/Part:BBa_B0012:Design
sAC Protein secuence can be found in http://www.genome.jp/dbget-bin/www_bget?bmy:Bm1_53800
Terminator B0030 can be found in http://partsregistry.org/Part:BBa_B0030:Design
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23101 RBS-3\Weak BBa_B0032 RBS BBa_J23101 RBS-3\Weak BBa_B0032 RBS BBa_J23101 RBS-3\Weak BBa_B0032 RBS BBa_B0015 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_B0015 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_B0015 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop | 1,35 44,56 44,56 50,53 65,99 108,120 108,120 114,117 129,163 172,184 172,184 178,181 1056,1184 1056,1135 1067,1110 1144,1184 1151,1170 1171,1184 1177,1177 1193,1321 1193,1272 1204,1247 1281,1321 1288,1307 1308,1321 1314,1314 1330,1458 1330,1409 1341,1384 1418,1458 1425,1444 1445,1458 1451,1451 | engineered_region feature/BioBrick ribosome_entry_site feature/rbs engineered_region feature/BioBrick ribosome_entry_site feature/rbs feature/BioBrick engineered_region feature/rbs ribosome_entry_site feature/BioBrick engineered_region feature/rbs ribosome_entry_site feature/BioBrick engineered_region ribosome_entry_site feature/rbs feature/BioBrick engineered_region ribosome_entry_site feature/rbs engineered_region feature/BioBrick engineered_region feature/BioBrick stem_loop feature/stem_loop feature/BioBrick engineered_region feature/stem_loop stem_loop feature/polya polyA_site feature/stop stop_codon engineered_region feature/BioBrick engineered_region feature/BioBrick stem_loop feature/stem_loop feature/BioBrick engineered_region stem_loop feature/stem_loop polyA_site feature/polya stop_codon feature/stop feature/BioBrick engineered_region feature/BioBrick engineered_region feature/stem_loop stem_loop engineered_region feature/BioBrick stem_loop feature/stem_loop feature/polya polyA_site feature/stop stop_codon |