Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ernst Bank
Date created: 2012-09-16 11:00:00
Date modified: 2015-05-08 01:13:38
M.ScaI Methyltransferase
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K874000_sequence (Version 1) |
Description
The M.ScaI protein is a type II methyltransferase (subtype beta) that recognises site on theDNA of the following sequence 5..AGTACT..3. It methylates this site at the 5th (Cytosine) nucleotide leaving ao an N4-methylcytosine (m4). This methylation type (m4) is not found in native E. coli nor is the recognition site methylated by any of E. coli's native methylation systems (Dam, Dcm).
This part also includes a flexible linker with incorporated myc-tag at the N terminus of the protein. This will allow for easier creation of fusion proteins and expression verification using westerblot (using the myc antibodies).
It can be assumed that M.ScaI (with linker) is expressed and folds properly in E. coli because its function has been verified by the iGEM Amsterdam 2012 team.
Notes
Contains no forbidden sites (according to the RFC-10 protocol).Contains no sites vulnerable to E. coli's native restriction systems.
Source
Natively found in Streptomyces caespitosus.Sequence obtained from REBASE.
Synthesized by IDT.