BBa_K874020

BBa_K874020 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K874020

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ernst Bank

Date created: 2012-09-18 11:00:00

Date modified: 2015-05-08 01:13:38

Myc-Linker (inc. start codon)

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_K874020_sequence (Version 1)

Description

Flexible linker with integrated Myc-Tag that can be used to create fusion proteins. This linker is used by van Steensel lab to create Transcriptionfactor Methyltransferase (Dam) fusion proteins and has thus been verified to have no or minimal impact on the function of either. This linker was also verified by iGEM Amsterdam 2012 to have no significant impact on the expression of its attached Methyltransferase (M. ScaI).

Notes

Since this part was synthesized it was checked for any Forbidden and to be Avoided restriction sites (RFC-10).

Source

Sequence obtained from van Steensel Lab and was part of pC-MBD2_3-Myc-Dam construct.
Synthesized as part of construct intermediate ???? by IDT.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start	4,6	start_codon, feature/start
Myc-Tag	22,51	feature/tag, tag
Linker	4,60	feature/protein, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K874020/1

Member of these Collections

• iGEM Parts Registry