BBa_K875000

BBa_K875000 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K875000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Federico Colombo, Elisa Clagnan

Date created: 2012-09-22 11:00:00

Date modified: 2015-05-08 01:13:38

B-Glucosidase

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K875000_sequence (Version 1)

Description

This part is composed by J23100-B0034-β-glucosidase-B0015. When this part is expressed in bacteria, they become able to metabolize cellobiose converting it in two molecules of glucose.

Notes

We made a PCR on Bba_K392008 by using the following primers:
β-Glucosidase_Fw
GCATGAATTCGCGGCCGCTTCTAGATGACCACCACGCGCCCCTC
β-Glucosidase_Rev
GCATCTGCAGCGGCCGCTACTAGTATCAGGGCTGGTAGGTCGCGG

Source

We made this BioBrick using others parts already present in the Registry of standard parts.

Components

Access	Instance	Definition
public	BBa_J23100	BBa_J23100
public	BBa_B0034	BBa_B0034
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23100	1,35	BBa_J23100
BBa_B0034	44,55	BBa_B0034
BBa_B0015	64,192	BBa_B0015

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K875000/1

Member of these Collections

• iGEM Parts Registry