BBa_K875001

BBa_K875001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K875001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Federico Colombo, Elisa Clagnan

Date created: 2012-09-22 11:00:00

Date modified: 2015-05-08 01:13:38

T5 Cumate Operator

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K875001_sequence (Version 1)

Description

This promoter is composed by phage T5 promoter and Cumate operator. It is repressed in the presence of CymR protein which binds the Cumate Operator.

Notes

We added the standard iGEM prefix and suffix in order to clone it into the standard iGEM vector pSB1C3

Source

1) The cumate gene-switch: a system for regulated expression in mammalian cells
Alaka Mullick, Yan Xu, Ren?? Warren, Maria Koutroumanis, Claire Guilbault, Sophie Broussau, F??lix Malenfant, Lucie Bourget, Linda Lamoureux, Rita Lo, Antoine W Caron, Amelie Pilotte and Bernard Massie ( BMC Biotechnology 2006, 6:43 doi:10.1186/1472-6750-6-43 )

2. Novel, Versatile, and Tightly Regulated Expression System for Escherichia coli Strains
Young J. Choi, Lyne Morel, Teffanie Le Fran??ois Denis Bourque, Lucie Bourget, Denis Groleau, Bernard Massie and Carlos B. M??guez (Applied and Environmental Microbiology, Aug. 2010, p. 5058???5066 Vol.76 doi:10.1128/AEM.00413-10)

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K875001/1

Member of these Collections

• iGEM Parts Registry