Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marija Drikic, Giorgio Gargari
Date created: 2012-09-22 11:00:00
Date modified: 2015-05-08 01:13:38
T5LacO-LPP-OmpA-SIP (scFv 54.6 + alpha isotype CH3 antinorovirus)-6HIS-TT
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K875005_sequence (Version 1) |
Description
This construct is designed for the expression of an already descibed engeneered antinorovirus (NoV) monoclonal antibody (mAb 54.6) in fusion with LPP-OmpA.The antibody is expressed in a small immuno protein (SIP) format containing light (VL) and heavy (VH) variable domains separeted by a flexible peptide linker and constant domain (CH3) of heavy chain of human immunoglobulin A (IgA). It has already been reported that the scFv 54.6 (which makes part of SIP) binds a native recombinant NoV particles (VLPs) and inhibits VLP interaction with cells. Alpha isotype CH3 domain homodimerizes confering bivalent binding properties to the antibody. LPP-OmpA functions as a leader sequence and an anchor to display the SIP on the bacterial surface.
The construct consistes of T5 Lac Operator (Bba_K875002), ribosomal binding site, LPP-OmpA-SIP 54.6 antinorovirus, Hystidine tag (6HIS), Terminator (B0015).
Notes
This coding sequence was optimized for the expression in prokaryotic cells. It has a Histidine tag.Source
Gene synthesis.Reference:
1.???Transport and anchoring of 8-lactamase to the external surface of
Escherichia coli??? J. A. Francisco, C. F. Earhart and G. Georgiou.
Proc. Natl. Acad. Sci. USA Vol. 89, pp. 2713-2717, April 1992 Biochemistry.
2. ???Recombinant norovirus-specific scFv inhibit virus-like particle binding to
cellular ligands??? K. Ettayebi and M. E. Hardy.
Published: 31 January 2008 in Virology Journal 2008, 5:21?