BBa_K875020

BBa_K875020 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K875020
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Federico Colombo, Elisa Clagnan, Marija Drikic, Giorgio Gargari, Giulia Corso, Sara Samari
Date created: 2012-09-22 11:00:00
Date modified: 2015-05-08 01:13:38

β
-Glucosidase



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K875020_sequence (Version 1)

Description

This part is composed by J23100-B0034-β-glucosidase-B0015. When this part is expressed in bacteria, they become able to metabolize cellobiose converting it in two molecules of glucose.

Notes

We made a PCR on Bba_K392008 by using the following primers: β-Glucosidase_Fw GCATGAATTCGCGGCCGCTTCTAGATGACCACCACGCGCCCCTC β-Glucosidase_Rev GCATCTGCAGCGGCCGCTACTAGTATCAGGGCTGGTAGGTCGCGG

Source

We made this BioBrick using others parts already present in the Registry of standard parts.

Sequence Annotation Location Component / Role(s)
BBa_J23100
BBa_B0034
B-Glucosidase
BBa_B0015
1,35
44,55
62,1516
1525,1653
feature/promoter promoter
ribosome_entry_site feature/rbs
CDS feature/cds
feature/stem_loop stem_loop
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K875020/1