Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ting Chang
Date created: 2012-09-08 11:00:00
Date modified: 2015-05-08 01:13:40
Plac(promoter)+aRBS(B0034)+zif268+alsS+a RBS(B0034)+PBSII+ilvC
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K887071_sequence (Version 1) |
Description
This is the first part of our low temperature release system biobrick. It can make e.coli pass the pathway : glucose->pyruvate->2-acetolactate->2,3-Dihydroxy-isovalerate.Plac is the promoter gene.B0034 is the ribosome binding site.zif268 and PBSII are zinc fingers which help enzymes,alsS and ilvC,to bind the specific DNA sequence and work like factorys gathering together to work efficiently.AlsS is the enzyme which is responsible for converting pyruvate into 2-acetolactate.IlvC is responsible for converting 2-acetolactate into 2,3-Dihydroxy-isovalerate.Notes
We had been afraid that it is too long to connect all of them become one part of our biobrick.Fortunately,we are successful for connecting all of them with no problems.Source
We advance the 2011 igem NCTU_Formosa Team's idea to produce isobutanol more efficiently and successfully.| Access | Instance | Definition |
| public public public public public public public | BBa_B0010 BBa_B0034 BBa_K165006 BBa_K539713 BBa_B0034 BBa_K323107 BBa_K539711 | BBa_B0010 BBa_B0034 BBa_K165006 BBa_K539713 BBa_B0034 BBa_K323107 BBa_K539711 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0010 BBa_B0034 BBa_K165006 BBa_K539713 BBa_B0034 BBa_K323107 BBa_K539711 | 1,80 89,100 109,360 367,2082 2091,2102 2111,2398 2405,3880 | BBa_B0010 BBa_B0034 BBa_K165006 BBa_K539713 BBa_B0034 BBa_K323107 BBa_K539711 |