BBa_K888004

BBa_K888004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K888004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: P. Quintero-Cadena and constructed by J.G. Rodriguez-Ceja
Date created: 2012-09-24 11:00:00
Date modified: 2015-05-08 01:13:40

Biphasic switch part



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K888004_sequence (Version 1)

Description

The biphasic switch may be used to control both expression and repression of pRM through a single input, which must control cI protein concentration. Furthermore, if repressor cI434 is placed under pRM's control along with the gene of interest, this switch may be coupled with the modified promoter pRM434 (BBa_I12006), which is stimulated by cI but repressed by cI434; hence resulting in a two-state switch controlled through a single input.

Notes

Design by P. Quintero-Cadena and Constructed by J.G. Rodriguez-Ceja

Source

This part is composed by pRM promoter (K556001), RBS (B0034), cI434 coding sequence (K566011) and GFP coding sequence (K145015).

igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K888004/1