BBa_K888006

BBa_K888006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K888006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: J. F. Quijano

Date created: 2012-09-24 11:00:00

Date modified: 2015-05-08 01:13:40

Mocr (solubilization tag)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K888006_sequence (Version 1)

Description

Mocr is a monomeric version of the OCR protein of the T7 bacteriophage, which has shown solubility properties to be used as solubilization tag in order to avoid inclusion bodies formation of fusion proteins (DelProposto et al. 2008).

DelProposto et al. (2008) Mocr: A novel fusion tag for enhancing solubility that is compatible with structural biology applications. Protein Expr Purif. 63(1): 40-9

Notes

This part has the following prefix sites, EcoRI and BglII and as suffix sites, BamHI and SpeI. Through this way, it is possible to obtain fusion proteins in frame via BglII and BamHI sites, and also enables the use of Standard Assembly 10 vectors.

Source

T7 Bactriophage

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
cds	1,363	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K888006/1

Member of these Collections

• iGEM Parts Registry