BBa_K895005

BBa_K895005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K895005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frank Sargent
Date created: 2012-09-22 11:00:00
Date modified: 2015-05-08 01:13:41

Salty_VgrG-CD27_endolysin



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K895005_sequence (Version 1)

Description

This is a coding part comprising the full open reading frame of Salmonella enterica LT2 vgrG gene (also known as STM0289 or tssI) linked via an haemagglutinin eptitope tag to a synthetic gene encoding an endolysin from the CD27 bacteriophage. The CD27 endolysin is believed to have high specificity against the cell wall of Clostridium difficile.

Notes

None.

Source

The vgrG section is derived from BBa_K895002, which was used as a PCR template, and the endolysin section is derived from a synthetic gene designed by Neil Fairweather's group at Imperial College London.

Sequence Annotation Location Component / Role(s)
vgrG
HA tag
endolysin
1,2187
2188,2214
2221,2793
feature/cds CDS
feature/tag tag
feature/cds CDS
igem#experience
Issues
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/myers
 
synbiohub#ownedBy
user/james
 
synbiohub#topLevel
BBa_K895005/1