Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andreas Kj??r
Date created: 2012-09-25 11:00:00
Date modified: 2015-05-08 01:13:42
Doubble ribosomal binding site (FFT) (shine-dalgarno and kozak sequence)
| Types | DnaRegion |
| Roles | RBS ribosome_entry_site |
| Sequences | BBa_K899009_sequence (Version 1) |
Description
This is the ribosomal binding site(s) we used in front of our fructan:fructan fructosyltransferase (FFT. It consists of the kozak sequence originally from the gene when we extracted it from the jerusalem artichoke (Helianthus Tuberosus), and the shine dalgarno sequence aggaggt.Notes
the shine dalgarno is placed 6 bp from the startcodon (optimal distance) and kozak is 4 of theese bp right before start codon.Source
jerusalem artichoke (Helianthus Tuberosus) and modified with bacterial shine-dalgarno sequence| Sequence Annotation | Location | Component / Role(s) |
| Kozak sequence Shine-Dalgarno sequence | 10,13 1,7 | feature/misc sequence_feature feature/misc sequence_feature |