BBa_K899009

BBa_K899009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K899009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andreas Kj??r

Date created: 2012-09-25 11:00:00

Date modified: 2015-05-08 01:13:42

Doubble ribosomal binding site (FFT) (shine-dalgarno and kozak sequence)

Details

• Types: DnaRegion
• Roles: RBS; ribosome_entry_site
• Sequences: BBa_K899009_sequence (Version 1)

Description

This is the ribosomal binding site(s) we used in front of our fructan:fructan fructosyltransferase (FFT. It consists of the kozak sequence originally from the gene when we extracted it from the jerusalem artichoke (Helianthus Tuberosus), and the shine dalgarno sequence aggaggt.

Notes

the shine dalgarno is placed 6 bp from the startcodon (optimal distance) and kozak is 4 of theese bp right before start codon.

Source

jerusalem artichoke (Helianthus Tuberosus) and modified with bacterial shine-dalgarno sequence

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Kozak sequence	10,13	feature/misc, sequence_feature
Shine-Dalgarno sequence	1,7	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K899009/1

Member of these Collections

• iGEM Parts Registry