BBa_K900000

BBa_K900000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K900000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Austin Jones
Date created: 2012-09-26 11:00:00
Date modified: 2015-05-08 01:13:42

PAmCherry (photoactivatable red fluorescent protein)



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_K900000_sequence (Version 1)

Description

PAmCHERRY is a photoactivatable red fluorescent protein. PAmCherry fluoresces when exposed to 350???400 nm light while does not fluoresce at other wavelengths. It is used a reporter tag: by expressing in cells or localizing to sub-cellular locations, one can gain spatial information within the cell by track organelles, proteins, and the whole cell itself. One can visualize the photoactivated PAmCherry with the same filter sets used for other red fluorescent proteins.

Notes

Codon optimization for yeast and internal restriction site removal to be compatible with our Golden Gate cloning scheme.

Source

Synthesized in house. Non-genomic DNA sequence. Mutated version of RFP.

igem#experience
Works
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K900000/1