Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lisa Oberding, Ali Honarmand
Date created: 2012-09-30 11:00:00
Date modified: 2015-05-08 01:13:43
DBT-sulfone monooxygenase (DszA) from R. erythropolis
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K902050_sequence (Version 1) |
Description
A monooxygenase enzyme involved in the 4S desulfurization pathway. This protein encoded by this sequence carries out the third step in the pathway, converting DBT-sulfone to 2-hydroxybiphenyl-2-sulfinic acid through addition of an oxygen to the sulfur heteroatom. FMNH2 is consumed by this reaction.Notes
4 internal PstI cut-sites had to be mutated with site-directed mutagenesis.Source
Obtained from PCR amplification from a plasmid found in an environmental isolate of R. erythropolis shown to be an active desulfurizer.| Sequence Annotation | Location | Component / Role(s) |
| start G->A G->A G->A G->A DszA stop | 1,3 639,639 678,678 1011,1011 1266,1266 1,1362 1360,1362 | feature/start start_codon sequence_alteration feature/mutation sequence_alteration feature/mutation sequence_alteration feature/mutation sequence_alteration feature/mutation CDS feature/cds feature/stop stop_codon |