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dszB

BBa_K902052 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K902052

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lisa Oberding, Ali Honarmand

Date created: 2012-09-30 11:00:00

Date modified: 2015-05-08 01:13:43

HBPS desulfinase (DszB) from R. erythropolis (mutated for improved activity)

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Details

Types	DnaRegion
Roles	Coding CDS
Sequences	BBa_K902052_sequence (Version 1)

Description

DszB - HBPS desulfinase which carries out the last step of the 4S pathway, converting 2-hydroxybiphenyl-2-sulfinic acid (HBPS) to 2-hydroxybiphenyl. The sequence has been mutated Y63F to increase enzyme activity.

Notes

Mutations of internal PstI and NotI cut sites in order to make biobrick compatible, as well as an added mutation of Y63F in the protein to increase its activity.

Source

Amplified from a plasmid in an environmental isolate of Rhodococcus shown to be an active desulfurizer.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
DszB start Y63F G->A G->A stop	1,1098 1,3 187,189 339,339 1026,1026 1096,1098	CDS feature/cds feature/start start_codon feature/mutation sequence_alteration feature/mutation sequence_alteration feature/mutation sequence_alteration feature/stop stop_codon