| Types | DnaRegion
|
| Roles | Protein_Domain
polypeptide_domain
|
| Sequences | BBa_K906004_sequence (Version 1)
|
Description
This is a tag which is added to the C terminus of a protein in order to aid in the identification of the protein in vivo, as well as to simplify the purificaiton process of the protein. The V5 epitope bind the Anti-V5 antibody. The histidine tag allows for purification using a nickel column. It was sourced from the Invitrogen pBAD/Thio-TOPO, which enables the expression of PCR amplified DNA. This protein also includes a linker to attach the V5 and histidine regions with the protein that is being expressed.
Notes
The sequence includes a stop codon (TGA) making it useful as a C-terminal tag.
Source
This part is a component of the vector included with the Invitrogen pBAD/Thio-TOPO kit.
