| Types | DnaRegion
|
| Roles | Translational_Unit
engineered_region
|
Description
This translational unit is designed to constitutivly express Zeaxanthin 7,8-cleavage dioxygenase (ZCD) in synechocystis. PsbA2 can be removed from this part and utilized with an alternative part by using NdeI to cut between the end of psbA2 and the start codon of ZCD. The ORF to be attached to this promoter should be made to have the same restriction enzyme site (possibly added by PCR) at its 5' end. This will allow for spacing between the regulatory features of psbA2 and the ORF to parallel that of the native use of the promoter.
Notes
PsbA2 is from the genomic sequence preceding the photosystem II D1 protein coding region in Synechocystis spp. 6803. The modified version of ZCD has been codon optimized for the species Synechocystis 6803.
Source
The point mutation (A->T) at the 200th base enables the use of NdeI as mentioned on the main page.
