Bxb1_Int

BBa_K907000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K907000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dong-hui Choe, Soo-in Lee
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:44

Mycobacterium Phage Bxb1 gp35, DNA integrase



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K907000_sequence (Version 1)

Description

This part is the protein coding sequence which encodes DNA integrase of Mycobacterium phage Bxb1.
The Bxb1 integrase is a DNA recombinase, more precisely a member of serine integrase family. It recognizes specific sequences, called attB and attP, and then integrate, invert, or excise depend on orientations of recognition sequences.
We used this integrase to invert specific sequence contained in plasmid. This protein is well expressed in E.coli(strain MG1655).
When it inverts DNA, the attB and attP sequences are changed into attL and attR, as common DNA recombinases do. Another protein called Bxb1 excisionase interacts with integrase and that complex flips back inverted DNA into original sequence regenerating attB and attP sequences.

Notes

Internal restriction endonuclease site is changed(two PstI sites, one XbaI sites) without changing amino acid sequence.

Source

This part is derived by Mycobacterium phage Bxb1. And synthesized by Bioneer.
http://www.ncbi.nlm.nih.gov/protein/NP_075302.1

Sequence Annotation Location Component / Role(s)
Mycobacterium phage Bxb1 integrase CDS
1,1503
CDS feature/cds
igem#experience
None
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/myers
 
synbiohub#ownedBy
user/james
 
synbiohub#topLevel
BBa_K907000/1