Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stuart Bell
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:45
Fast Germination (B.subtilis)
| Types | DnaRegion |
| Roles | Regulatory promoter |
Description
This part upregulates an operon responsible for germination rate. Bacillus subtilis spores germinate in response L-Alanine. Up-regulation of the spoVA operon increases germination rate in response to L-Alanine. The promoter for the B.subtilis sspB gene (PsspB) is more active than the endogenous spoVA promoter. It is also active during germination. Expression of the spoVA operon under PsspB increases the germination rate.This construct consists mainly of the sspB promoter followed by the first 354bp of the spoVA operon (first 354bp of the spoVAA gene). Since B.subtilis exhibits accurate and efficient homologous recombination, a single cross-over event between the spoVAA region in the BioBrick and the endogenous spoVAA sequence inserts the sspB promoter upstream of the spoVA operon.
Notes
The design of this BioBrick is based on a plasmid (pPS3393)from Wang et al. (2011)Wang, G., Yi, X., Li, Y.-Q., Setlow, P. Germination of individual Bacillus subtilis spores with alterations in the GerD and SpoVA proteins, which are important in spore germination(2011) J. Bacteriology, 193 (9), pp. 2301-2311.