BBa_K912007

BBa_K912007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K912007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grant Nicholas

Date created: 2012-09-27 11:00:00

Date modified: 2015-05-08 01:13:45

Pgad/GFP composite part

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K912007_sequence (Version 1)

Description

This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector meant to self lyse in high chloride and low pH conditions. The device is designed to fluoresce in the presence of high chloride concentrations. The Pgad promoter in front of the GFP is activated by the combination of high extracellular hydrogen ion concentration and the presence of high intracellular chloride ion concentration.

Notes

The Pgad part was not in the registry so the promoter had to be PCR'd out of an unknown vector sent by the CUHK 2011 team

Source

Pgad: CUHK 2011 team
GFP: Parts registry

Components

Access	Instance	Definition
public	BBa_K559011	Pgad
public	BBa_E0040	GFP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K559011	1,1251	Pgad
BBa_E0040	1258,1977	GFP

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K912007/1

Member of these Collections

• iGEM Parts Registry