BBa_K912033

BBa_K912033 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K912033

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grant Nicholas

Date created: 2012-10-02 11:00:00

Date modified: 2015-05-08 01:13:45

pH Inducible Lysis System(ns)

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K912033_sequence (Version 1)

Description

This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector meant to self lyse in high chloride and low pH conditions. The device is designed to lyse in the presence of high chloride concentrations. The Pgad promoter in front of the lysis cassette is activated by the combination of high extracellular hydrogen ion concentration and the presence of high intracellular chloride ion concentration.

Notes

None besides designing primers properly

Source

Lysis:registry
CP/RBS: registry
clc-ec1: PCR from genome
Pgad: PCR from vector from CUHK 2011 team

Components

Access	Instance	Definition
public	BBa_K912029	BBa_K912029
public	BBa_K912012	BBa_K912012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K912029	1,3044	BBa_K912029
BBa_K912012	3053,4515	BBa_K912012

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K912033/1

Member of these Collections

• iGEM Parts Registry