BBa_K914004

BBa_K914004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K914004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Dylan Iverson

Date created: 2012-09-19 11:00:00

Date modified: 2015-05-08 01:13:45

Pbad/AraC RBS LacI

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K914004_sequence (Version 1)

Description

Pbad/AraC-RBS-LacI

Used as part of the simple delay mechanism for bWARE suicide mechanism. When arabinose is no longer present, LacI will no longer be present to repress another plasmid that expresses a restriction enzyme under Plac.

Notes

Removed LVA tag from LacI. Intended for use on low copy plasmid.

Source

Modified from a part from Paris Bettencourt 2009 iGEM team.

Components

Access	Instance	Definition
public	BBa_I13458	BBa_I13458
public	BBa_I13453	BBa_I13453
public	BBa_B0034	BBa_B0034
public	BBa_K143033	LacI

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I13458	1,1114	BBa_I13458
BBa_I13453	1123,1252	BBa_I13453
BBa_B0034	1261,1272	BBa_B0034
BBa_K143033	1279,2364	LacI

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K914004/1

Member of these Collections

• iGEM Parts Registry