BBa_K914007

BBa_K914007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K914007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Denis Samuylov
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:46

Meganuclease I-SceI controlled by pBad



    • Details

    Types
    DnaRegion

    Roles
    Generator

    engineered_region

    Sequences BBa_K914007_sequence (Version 1)

    Description

    I-SceI homing endonuclease expression is controlled by pBad promoter. Expression is induced with IPTG if LacI is present in the cell. I-SceI doesn't have an LVA degradation tag.

    Notes

    The system is leaky and meganuclease cut RS with high efficiency even without pBad induction.

    Source

    Promoter pBad and RBS was taken from an iGEM distribution plate (BBa_I13453 and BBa_B0032). Meganuclease was amplified from the chromosome of SMR6316 E.Coli strain (Dr. Rosenberg).

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