BBa_K914007

BBa_K914007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K914007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Denis Samuylov

Date created: 2012-09-19 11:00:00

Date modified: 2015-05-08 01:13:46

Meganuclease I-SceI controlled by pBad

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K914007_sequence (Version 1)

Description

I-SceI homing endonuclease expression is controlled by pBad promoter. Expression is induced with IPTG if LacI is present in the cell. I-SceI doesn't have an LVA degradation tag.

Notes

The system is leaky and meganuclease cut RS with high efficiency even without pBad induction.

Source

Promoter pBad and RBS was taken from an iGEM distribution plate (BBa_I13453 and BBa_B0032). Meganuclease was amplified from the chromosome of SMR6316 E.Coli strain (Dr. Rosenberg).

Components

Access	Instance	Definition
public	BBa_I13453	BBa_I13453
public	BBa_B0032	BBa_B0032
public	BBa_K914006	BBa_K914006

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I13453	1,130	BBa_I13453
BBa_B0032	139,151	BBa_B0032
BBa_K914006	158,869	BBa_K914006

Other Properties

• igem#experience: Issues
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K914007/1

Member of these Collections

• iGEM 2018 Distribution Plate 6
• iGEM 2018 Distribution Plate 6 Well 4L
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM 2019 Distribution Plate 4
• iGEM 2019 Distribution Plate 4 Well 4L
• iGEM 2019 Part Library
• iGEM 2019 Plasmid pSB1C3
• iGEM Parts Registry