Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Elitsa Peeva
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:46
nfsI nitroreductase gene from Enterobacter cloacae
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K917004_sequence (Version 1) |
Description
This is the gene (nfsI) from Enterobacter cloacae which encodes nitroreductase. This is an enzyme which reduces nitrogen containing compounds. Nitroreductases were implemented in convering nitro drugs such as metronidazole into their active form which is essential part of their toxicity.Expression of this gene in the presence of metronidazole leads to growth inhibition. This gene could possibly could be counter selectable marker.Notes
This part includes the native ribosome binding site.Source
Enterobacter cloacae| Sequence Annotation | Location | Component / Role(s) |
| start codon stop codon RBS | 15,17 666,668 5,11 | start_codon feature/start feature/stop stop_codon feature/rbs ribosome_entry_site |