Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eric Pederson
Date created: 2012-09-30 11:00:00
Date modified: 2015-05-08 01:13:46
T7 RNAP + IPTG->PoPs (Mutant III)
| Types | DnaRegion |
| Roles | T7_RNA_Polymerase_Promoter T7 |
| Sequences | BBa_K921002_sequence (Version 1) |
Description
This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).Notes
There are three portions to the T7 promoter: the recognition site, the melting box (or TATA box) and the initation site. Mutations in these sequences caused differential behavior from the wild type.Source
The part consists of a sequences that resemble a T7 promoter and the lacO sequence found in E. coli.| Sequence Annotation | Location | Component / Role(s) |
| T7 Promoter LacO Initiation | 1,20 19,47 18,20 | promoter feature/promoter operator feature/operator sequence_feature feature/misc |