Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Potsdam Bioware 2012 iGEM
Date created: 2012-09-15 11:00:00
Date modified: 2015-05-08 01:13:46
AID with CMV promoter and hGH-polyadenylation signal sequence
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K929000_sequence (Version 1) |
Description
The BioBrick wtAID is an extended version of the existing AID BioBrick (BBa_K103001). It is built of 3 parts: CMV promoter, AID and hGH-polyA tail.AID:
AID is known to be responsible for somatic hypermutation and the class-switch recombination of immunoglobulin in B cells. This enzyme of 28 kDa originally occurs in B cells but does also show activity after transfection into CHO cells. AID induces the deamination of cytidine to uridine at actively transcribed single strand DNA. The replacement of cytidine by uridine leads to a mismatch during DNA replication and integrates a single base substitution predominantly in the immunoglobulin genes.
CMV promoter:
CMV stands for cytomegalovirus. The CMV promoter is commonly used due to its very strong activity, and effectivity in a broad range of cell types. The BioBrick is therefore improved via addition of the strong promoter.
hGH-polyA tail:
The polyA tail consists of multiple adenosine monophosphates. hGH-polyA tail is a significant part for the translation and stability of mRNA. In eukaryotes, polyadenylation is part of the process that produces mature messenger RNA (mRNA) for translation. It, therefore, forms part of the larger process of gene expression.
Notes
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It is a composite part consisting of 3 other Biobricks.| Sequence Annotation | Location | Component / Role(s) |
| CMV promoter BBa_I712004 NcoI BBa_K103001 AID AUG UGA hGH terminator BBa_K404108 | 1,654 1,654 663,668 663,1262 665,1262 665,667 1259,1262 1276,1754 1276,1754 | feature/promoter promoter engineered_region feature/BioBrick feature/dna sequence_feature feature/BioBrick engineered_region feature/cds CDS feature/start start_codon feature/stop stop_codon feature/polya polyA_site engineered_region feature/BioBrick |