Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Potsdam Bioware 2012 iGEM
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:46
modified AID+eGFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K929004_sequence (Version 1) |
Description
This is a composite part from the existing parts: modified_AID (BBa_929001) and eGFP (BBa_K404316). We used RFC 25 enzymes to create this part as a fusion of modified AID and eGFP (at the C-terminus of mod_AID). This part was not used to express the fusion protein. It is an usefull intermediate that can be combined with differnt promoters and terminators. We combined this part with CMV-promoter and polyA-hGH (poly adenylation signal sequence)--> see BBa_929002.Notes
We used RFC 25 to combine modified_AID (BBa_929001) and eGFP (BBa_K404316)Source
This is a composite part from the existing parts: modified_AID (BBa_929001) and eGFP (BBa_K404316). We used RFC 25 enzymes to create this part as a fusion of modified AID and eGFP (at the C-terminus of mod_AID).| Sequence Annotation | Location | Component / Role(s) |
| Kozak sequence BBa_K929001 ATG NLS sequence part of BBa_K103001 BBa_K404316 TAA AgeI | 1,15 1,581 9,11 16,34 35,581 582,1304 1302,1304 1296,1301 | feature/rbs ribosome_entry_site feature/BioBrick engineered_region start_codon feature/start sequence_feature feature/dna engineered_region feature/BioBrick engineered_region feature/BioBrick feature/stop stop_codon feature/dna sequence_feature |