BBa_K929102

BBa_K929102 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K929102
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Potsdam Bioware 2012 iGEM
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:46

Anti-GFP Nanobody with IgG kappa signal peptide and IgG1 Fc domain



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K929102_sequence (Version 1)

Description

This part represents an antibody unit composed of a IgG kappa signal peptide, an anti-GFP nanobody and an IgG1 Fc domain.

Notes

Part BBa_K929107 was used as template.

Source

Signal peptide: human Ig kappa chain V-I region taken from UniProt P01601[1-22], gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)
Nanobody: anti-GFP nanobody taken from Protein Data Bank(PDB: 3OGO), gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)
Fc domain: human immunoglobulin gamma-1 heavy chain constant region taken from GenBank: J00228.1 (UniProt: P01857)

Sequence Annotation Location Component / Role(s)
IgG kappa signal peptide
anti-GFP nanobody
IgG1 Fc domain
1,66
67,348
349,696
feature/misc sequence_feature
CDS feature/cds
feature/cds CDS
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K929102/1