BBa_K929102

BBa_K929102 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K929102

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Potsdam Bioware 2012 iGEM

Date created: 2012-09-23 11:00:00

Date modified: 2015-05-08 01:13:46

Anti-GFP Nanobody with IgG kappa signal peptide and IgG1 Fc domain

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K929102_sequence (Version 1)

Description

This part represents an antibody unit composed of a IgG kappa signal peptide, an anti-GFP nanobody and an IgG1 Fc domain.

Notes

Part BBa_K929107 was used as template.

Source

Signal peptide: human Ig kappa chain V-I region taken from UniProt P01601[1-22], gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)
Nanobody: anti-GFP nanobody taken from Protein Data Bank(PDB: 3OGO), gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)
Fc domain: human immunoglobulin gamma-1 heavy chain constant region taken from GenBank: J00228.1 (UniProt: P01857)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
IgG kappa signal peptide	1,66	feature/misc, sequence_feature
anti-GFP nanobody	67,348	CDS, feature/cds
IgG1 Fc domain	349,696	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K929102/1

Member of these Collections

• iGEM Parts Registry